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  Indian J Med Microbiol
 

Figure 5: Differential osteoblastic and adipocytic differentiation of BM-MSC and AD-MSC. (a) Representative ALP staining on day 10 induction of BM-MSC (left panel) or AD-MSC (right panel). (b) Quantification of ALP activity in BM-MSC versus AD-MSC induced into osteoblast for 10 days. Data are presented as mean ± standard error of the mean; n = 8 from two independent experiments. ***P < 0.0005. (c) Representative Nile Red staining of lipid filled adipocytes on day 7 induction of BM-MSC (left panel) or AD-MSC (right panel). Images were captured using EVOS FL Auto system (Thermo) using ×10 objective. (d) Nile red quantification on day 7 after adipocytic induction of BM-MSC and AD-MSC. Data are presented as mean ± standard error of the mean; n = 6 from two independent experiments. ***P < 0.0005. BM-MSC: Bone marrow-derived human stromal stem cell, AD-MSC: Adipose-derived human stromal stem cell, ALP: Alkaline phosphatase

Figure 5: Differential osteoblastic and adipocytic differentiation of BM-MSC and AD-MSC. (a) Representative ALP staining on day 10 induction of BM-MSC (left panel) or AD-MSC (right panel). (b) Quantification of ALP activity in BM-MSC versus AD-MSC induced into osteoblast for 10 days. Data are presented as mean ± standard error of the mean; <i>n</i> = 8 from two independent experiments. ***<i>P</i> < 0.0005. (c) Representative Nile Red staining of lipid filled adipocytes on day 7 induction of BM-MSC (left panel) or AD-MSC (right panel). Images were captured using EVOS FL Auto system (Thermo) using ×10 objective. (d) Nile red quantification on day 7 after adipocytic induction of BM-MSC and AD-MSC. Data are presented as mean ± standard error of the mean; <i>n</i> = 6 from two independent experiments. ***<i>P</i> < 0.0005. BM-MSC: Bone marrow-derived human stromal stem cell, AD-MSC: Adipose-derived human stromal stem cell, ALP: Alkaline phosphatase